Reproduction Abstracts

Granulocyte colony-stimulating factor (G-CSF) enhances the proliferation, differentiation and survival of cells. In addition, G-CSF is a non-invasive biomarker of human oocyte developmental competence for embryo implantation. In human follicle G-CSF concentration increased during the ovulatory. At present experiment, the cumulus oocyte complexes (COCs) were aspirated from superficial follicles (1–3 and 3–6 mm). COCs from small and medium follicle were matured in prot...

The carboxyethylgermaniumsesquioxide (Ge-132) is an organogermanium compound known to have anti-oxidative effect. In this study, we examined the effect of Ge-132 on in vitro maturation (IVM) of porcine oocytes analyzing intracellular glutathione (GSH) and reactive oxygen species (ROS) levels, mRNA expression levels and subsequent embryonic development after in vitro fertilization (IVF) and parthenogenetic activation (PA). After 40 h of IVM, intracellular GSH ...

Ganglioside is an acidic glycosphingolipid with sialic acid residues. The b-series ganglioside GT1b was reported that suppressing damage of mtDNA by reactive oxygen species (ROS) in mouse brain. The purpose of this study is to investigate the effect of exogenous addition of GT1b on in vitro maturation (IVM) of porcine oocytes and to confirm the related bradykinin 2 receptors (B2R). GT1b were treated on IVM that concentration was 0 (control), 5, 10 and 20 nM. After IVM...

For developmental competence of oocyte derived from small follicle (~3 mm in diameter SF), pre-IVM system was developed for in vitro culturing SF. The purpose of this study is to establish the optimal phase and concentration for exogenous addition of pituitary adenylate cyclase-activating peptide (PACAP) on pre-IVM. To establish the appropriate phase for pre-IVM, we assessed nuclear status according to culture duration. The result of the nuclear stage assessment of th...

The purpose of this study is the effects of growth differentiation factor 8 (GDF8) and SB431542 (SB) on porcine oocytes in vitro maturation (IVM), subsequent embryonic development after in vitrofertilization (IVF). We were investigated the effect of GDF8 and SB treatment during IVM on nuclear maturation, intracellular glutathione (GSH), reactive oxygen species (ROS) levels, analyzed specific gene transcription and translation levels in cumulus cells after IVM, and emb...

Introduction: Lysophosphatidic acid (LPA) is a signaling molecule derived from phospholipid known to have biological activities such as stimulating proliferation and differentiation. The purpose of this study was to investigate the effect of LPA on IVM of porcine oocytes and subsequent embryonic development after IVF and parthenogenetic activation (PA).Methods: We examined nuclear maturation, intracellular GSH and ROS levels and subsequent embryonic deve...

Pigs provide outstanding models of human genetic diseases due to the striking similarities to human anatomy, physiology and genetics. Pig induced pluripotent stem cells (piPSCs) have been generated for several years but the cloning efficiency using unsynchronized piPSCs was extremely low. Here, we reported a method to produce diploid cloned embryos from piPSCs which were synchronized to metaphase. The piPSCs cell line was established using a drug-inducible system and exhibit s...

Transgenic (TG) pigs are currently regarded as an important animal model for various biomedical researches including a disease modeling and a regenerative medicine. We tried to develop a pig astrocyte-specific CreERT2-LoxP recombination system as a part of TG pig model of brain tumor arisen from astrocytic cell lineage. We designed two vector systems one retains pig glial fibrillary acidic protein (GFAP) promoter-CreERT2 transgene and the other has GFP ge...